Tissue fusion over nonadhering surfaces.
نویسندگان
چکیده
Tissue fusion eliminates physical voids in a tissue to form a continuous structure and is central to many processes in development and repair. Fusion events in vivo, particularly in embryonic development, often involve the purse-string contraction of a pluricellular actomyosin cable at the free edge. However, in vitro, adhesion of the cells to their substrate favors a closure mechanism mediated by lamellipodial protrusions, which has prevented a systematic study of the purse-string mechanism. Here, we show that monolayers can cover well-controlled mesoscopic nonadherent areas much larger than a cell size by purse-string closure and that active epithelial fluctuations are required for this process. We have formulated a simple stochastic model that includes purse-string contractility, tissue fluctuations, and effective friction to qualitatively and quantitatively account for the dynamics of closure. Our data suggest that, in vivo, tissue fusion adapts to the local environment by coordinating lamellipodial protrusions and purse-string contractions.
منابع مشابه
Minimally invasive sacroiliac joint fusion.
What are the benefi ts of minimally invasive sacroiliac joint fusion? Frank M. Phillips, MD: Traditionally, fusion of the sacroiliac joint has involved open exposure of the joint with direct decortication of the articular surfaces, followed by bone grafting with supplemental fixation. Minimally invasive approaches to sacroiliac joint fusion aim to reduce the morbidity associated with open appro...
متن کاملHuman Mesenchymal Stem Cell Morphology and Migration on Microtextured Titanium
The implant used in spinal fusion procedures is an essential component to achieving successful arthrodesis. At the cellular level, the implant impacts healing and fusion through a series of steps: first, mesenchymal stem cells (MSCs) need to adhere and proliferate to cover the implant; second, the MSCs must differentiate into osteoblasts; third, the osteoid matrix produced by the osteoblasts ne...
متن کاملGrowing coral larger and faster: micro-colony-fusion as a strategy for accelerating coral cover
Fusion is an important life history strategy for clonal organisms to increase access to shared resources, to compete for space, and to recover from disturbance. For reef building corals, fragmentation and colony fusion are key components of resilience to disturbance. Observations of small fragments spreading tissue and fusing over artificial substrates prompted experiments aimed at further char...
متن کاملPEEK Versus Ti Interbody Fusion Devices: Resultant Fusion, Bone Apposition, Initial and 26-Week Biomechanics.
STUDY DESIGN Comparative evaluation of in vitro and in vivo biomechanics, resulting fusion and histomorphometric aspects of polyetheretherketone (PEEK) versus titanium (Ti) interbody fusion devices in an animal model with similar volumes of bone graft. OBJECTIVE Identify differences in the characteristics of fusion and biomechanics immediately following implantation (time 0) and at 26 weeks w...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- Proceedings of the National Academy of Sciences of the United States of America
دوره 112 31 شماره
صفحات -
تاریخ انتشار 2015